What makes this process so useful is that there is very little noise associated with the ion tags. The TOF part of CyTOF refers to the time it takes for these particles to fly to the detector. This is done within a magnetic field so that the particles move across the magnetic field to a detector at a rate proportional to the size of the particle the lighter the particle, the faster it moves. Cells labeled with these antibody-isotope conjugates are then vaporized, ionizing the elements. In a mass cytometry experiment instead of tagging antibodies with fluorescent molecules, the tags are isotopes of any element that is not found naturally in the biological system under study. The name is an abbreviation for ‘cytometry Time Of Flight’, and the machine is used to conduct cytometry experiments on a much larger scale than can be achieved with a traditional fluorescence based cytometer. The CyTOF is a mass cytometer produced by DVS Sciences. The CyTOF is a Time of Flight based mass spectrometer that produces FCS files as an output.
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